7VP6
Structure of a transcription factor and DNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-06 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.1000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 26.628, 51.634, 71.031 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.260 - 2.570 |
Rwork | 0.184 |
R-free | 0.26510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7vp3 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.812 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.260 | 2.661 |
High resolution limit [Å] | 2.570 | 2.570 |
Rmerge | 0.088 | 0.265 |
Number of reflections | 3381 | 305 |
<I/σ(I)> | 26.39 | |
Completeness [%] | 99.1 | |
Redundancy | 11.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 60% v/v Tacsimate pH 7.0 |