7VP5

Structure of a transcription factor and DNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-17
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.08
Spacegroup name	P 1 21 1
Unit cell lengths	78.766, 76.896, 83.078
Unit cell angles	90.00, 113.85, 90.00

Refinement procedure

Resolution	29.100 - 2.992
Rwork	0.209
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7vp3
RMSD bond length	0.009
RMSD bond angle	1.004
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.100	3.098
High resolution limit [Å]	2.992	2.992
Rmerge	0.165	0.766
Number of reflections	18354	1508
<I/σ(I)>	8.8
Completeness [%]	97.8
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	100mM Tris pH 8.0 and 20% w/v PEG 4000