7VP4
Structure of a transcription factor and DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-17 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.603, 76.930, 83.227 |
| Unit cell angles | 90.00, 113.73, 90.00 |
Refinement procedure
| Resolution | 29.160 - 3.040 |
| Rwork | 0.211 |
| R-free | 0.26470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7vp3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.086 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.160 | 3.149 |
| High resolution limit [Å] | 3.040 | 3.040 |
| Rmerge | 0.100 | 0.462 |
| Number of reflections | 17509 | 1650 |
| <I/σ(I)> | 14.46 | |
| Completeness [%] | 97.1 | |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 100mM Sodium HEPES pH 7.0, 10% w/v PEG 4000 and 10% v/v 2-Propanol |
