7VP3
Structure of a transcription factor and DNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-11 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.219, 112.784, 156.842 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.170 - 3.003 |
Rwork | 0.198 |
R-free | 0.24640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7vp2 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.165 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.170 | 3.110 |
High resolution limit [Å] | 3.003 | 3.003 |
Rmerge | 0.260 | 0.834 |
Rmeas | 0.265 | |
Number of reflections | 25799 | 1836 |
<I/σ(I)> | 19.26 | |
Completeness [%] | 93.3 | |
Redundancy | 25.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 100 mM Sodium citrate/Citric acid pH 5.5, 20% w/v PEG 4000 and 10% v/v 2-Propanol |