7VP1
Structure of a transcription factor and DNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 68.123, 68.123, 210.819 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.210 - 2.902 |
R-factor | 0.1838 |
Rwork | 0.178 |
R-free | 0.23850 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.941 |
Data scaling software | XDS |
Phasing software | SHELXCD |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.210 | 3.006 |
High resolution limit [Å] | 2.902 | 2.902 |
Rmerge | 0.117 | 0.615 |
Rmeas | 0.119 | 0.623 |
Rpim | 0.019 | 0.099 |
Number of reflections | 6982 | 653 |
<I/σ(I)> | 38.48 | |
Completeness [%] | 99.5 | |
Redundancy | 20 | |
CC(1/2) | 0.999 | 0.975 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M Potassium sodium tartrate tetrahydrate, 20% w/v Polyethylene glycol 3350 |