7VP0
Crystal structure of P domain from norovirus GI.9 capsid protein.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2017-05-12 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 170.950, 103.550, 76.910 |
Unit cell angles | 90.00, 116.66, 90.00 |
Refinement procedure
Resolution | 48.040 - 2.100 |
R-factor | 0.2389 |
Rwork | 0.237 |
R-free | 0.26880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4rdj |
RMSD bond length | 0.008 |
RMSD bond angle | 0.956 |
Data scaling software | XDS (VERSION Nov 1, 2016 BUILT=20170215) |
Phasing software | PHASER (2.7.17) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.040 | 2.230 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 134729 | 21576 |
<I/σ(I)> | 4.44 | |
Completeness [%] | 98.1 | |
Redundancy | 1.95 | |
CC(1/2) | 0.980 | 0.368 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293.15 | 0.10M BICINE (pH 9), 1.70M Magnesium chloride |