7VOV

The crystal structure of human forkhead box protein in complex with DNA 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-20
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9795
Spacegroup name	P 31 2 1
Unit cell lengths	98.600, 98.600, 64.730
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.766 - 3.150
R-factor	0.2588
Rwork	0.248
R-free	0.27500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2c6y
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.766	19.766	3.330
High resolution limit [Å]	3.140	9.270	3.140
Rmerge	0.066	0.023	0.376
Rmeas	0.068	0.023	0.387
Total number of observations	129588
Number of reflections	6586	282	1038
<I/σ(I)>	37.42	130.99	6.54
Completeness [%]	99.8	97.2	99.3
Redundancy	19.676	18.117	18.53
CC(1/2)	1.000	1.000	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	6% ethylene glycol, 0.1 M Citric acid pH 3.5, and 15% PEG 6000