7VOH

The a-glucosidase QsGH13 from Qipengyuania seohaensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-02
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	58.815, 129.918, 161.304
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.280 - 2.197
Rwork	0.218
R-free	0.27380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6aav
RMSD bond length	0.007
RMSD bond angle	1.377
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.330
High resolution limit [Å]	2.197	2.200
Rmerge	0.115	0.246
Number of reflections	63387	19182
<I/σ(I)>	14.36
Completeness [%]	99.4	97.7
Redundancy	6.95	6.14
CC(1/2)	0.993	0.932

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	0.1 M Tris, pH 8.5, 0.2 MNaCl, 25% PEG 3350