7VOB

The crystal structure of a Radical SAM Enzyme BlsE involved in the Biosynthesis of Blasticidin S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97921
Spacegroup name	I 41 2 2
Unit cell lengths	164.293, 164.293, 157.324
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.176 - 2.092
R-factor	0.223417269732
Rwork	0.223
R-free	0.23706
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	0.963
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.570	2.210
High resolution limit [Å]	2.090	2.090
Number of reflections	63221	63221
<I/σ(I)>	10.5
Completeness [%]	99.9
Redundancy	26.7
CC(1/2)	0.996	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1 M 2-(N-morpholino) ethanesulfonic acid monohydrate (MES) at pH 5.66, 3.0 M NaCl