7VO6
High resolution crystal structure of human PSMD10 (Gankyrin)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
Synchrotron site | RRCAT INDUS-2 |
Beamline | PX-BL21 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2020-11-22 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9795 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 60.085, 60.085, 122.377 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.930 - 1.710 |
R-factor | 0.198 |
Rwork | 0.196 |
R-free | 0.23140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uoh |
RMSD bond length | 0.009 |
RMSD bond angle | 1.613 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.930 | 47.910 | 1.580 |
High resolution limit [Å] | 1.550 | 4.210 | 1.550 |
Rmeas | 0.108 | 0.042 | 2.693 |
Rpim | 0.035 | 0.014 | 1.217 |
Total number of observations | 18969 | 7113 | |
Number of reflections | 37545 | 2088 | 1616 |
<I/σ(I)> | 7.1 | 49.3 | 0.1 |
Completeness [%] | 99.0 | 100 | 86.2 |
Redundancy | 8.8 | 9.1 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 60% v/v Tacsimate pH 7.0 (Hampton Research) |