7VO6

High resolution crystal structure of human PSMD10 (Gankyrin)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	80
Detector technology	CCD
Collection date	2020-11-22
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9795
Spacegroup name	P 32 2 1
Unit cell lengths	60.085, 60.085, 122.377
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.930 - 1.710
R-factor	0.198
Rwork	0.196
R-free	0.23140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uoh
RMSD bond length	0.009
RMSD bond angle	1.613
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.930	47.910	1.580
High resolution limit [Å]	1.550	4.210	1.550
Rmeas	0.108	0.042	2.693
Rpim	0.035	0.014	1.217
Total number of observations		18969	7113
Number of reflections	37545	2088	1616
<I/σ(I)>	7.1	49.3	0.1
Completeness [%]	99.0	100	86.2
Redundancy	8.8	9.1	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	60% v/v Tacsimate pH 7.0 (Hampton Research)