7VO1

Structure of aminotransferase-substrate complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-02
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1.0
Spacegroup name	P 65 2 2
Unit cell lengths	113.740, 113.740, 290.652
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.090 - 2.990
R-factor	0.2231
Rwork	0.220
R-free	0.28350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2eo5
RMSD bond length	0.011
RMSD bond angle	1.719
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.040
High resolution limit [Å]	2.990	8.110	2.990
Rmerge	0.069	0.017	0.924
Rmeas	0.071	0.018	0.954
Rpim	0.016	0.004	0.232
Total number of observations	438249
Number of reflections	23423	1355	1114
<I/σ(I)>	5.9
Completeness [%]	100.0	99.4	99.9
Redundancy	18.7	16.4	16.2
CC(1/2)		1.000	0.906

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	PEG 3000, MgCl2, cacodylate