7VNW

Crystal Structure of tRNAVal from Sulfolobus Tokodaii(Dephosphorylated)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-09
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	32.560, 116.680, 56.140
Unit cell angles	90.00, 101.34, 90.00

Refinement procedure

Resolution	31.920 - 2.610
R-factor	0.2255
Rwork	0.224
R-free	0.26050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	D_1300024972
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	31.920	31.920	2.680
High resolution limit [Å]	2.610	11.670	2.610
Rmerge	0.111	0.037	1.151
Rmeas	0.121	0.040	1.254
Total number of observations	83923
Number of reflections	12524	140	948
<I/σ(I)>	14.52	37.57	1.88
Completeness [%]	99.7	97.2	99.6
Redundancy	6.701	6.586	6.325
CC(1/2)	0.998	0.999	0.596

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	80mM NaCl, 12mM Speremin-4HCl, 30% MPD, 40mM NaCacodylate pH 6.5