7VNV

Crystal Structure of tRNAVal from Sulfolobus Tokodaii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-25
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	32.650, 116.620, 57.238
Unit cell angles	90.00, 100.97, 90.00

Refinement procedure

Resolution	32.050 - 1.900
R-factor	0.1959
Rwork	0.195
R-free	0.21990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ivs
RMSD bond length	0.007
RMSD bond angle	1.013
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	32.050	32.050	1.950
High resolution limit [Å]	1.900	8.520	1.900
Rmerge	0.067	0.032	1.599
Rmeas	0.073	0.035	1.731
Total number of observations	217828
Number of reflections	32418	374	2324
<I/σ(I)>	15.65	43.63	1.53
Completeness [%]	98.7	98.7	96.8
Redundancy	6.719	6.048	6.764
CC(1/2)	0.998	0.998	0.602

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	80mM NaCl, 12mM Speremin-4HCl, 30% MPD, 40mM NaCacodylate pH 6.5