7VMZ
Crystal structure of a human Coronavirus 229E antibody C04 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2020-05-11 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.978 |
Spacegroup name | P 62 |
Unit cell lengths | 148.215, 148.215, 53.387 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.600 - 2.850 |
R-factor | 0.2496 |
Rwork | 0.248 |
R-free | 0.28540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6df2 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.950 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmeas | 0.289 | 1.204 |
Rpim | 0.065 | 0.323 |
Number of reflections | 15842 | 1570 |
<I/σ(I)> | 16.4 | |
Completeness [%] | 99.0 | 100 |
Redundancy | 18.7 | 13.2 |
CC(1/2) | 0.976 | 0.870 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 15% w/v Polyethylene glycol 3350, 0.01M Magnesium chloride hexahydrate, 0.005M Nickel(II) chloride hexahydrate, 0.1M HEPES pH 7.0 |