7VMY
Crystal structure of LimF prenyltransferase bound with GSPP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-10 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 |
Unit cell lengths | 47.280, 47.520, 61.190 |
Unit cell angles | 100.12, 104.69, 96.68 |
Refinement procedure
Resolution | 31.520 - 1.770 |
R-factor | 0.1717 |
Rwork | 0.170 |
R-free | 0.20980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5tty |
RMSD bond length | 0.013 |
RMSD bond angle | 1.419 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.520 | 1.830 |
High resolution limit [Å] | 1.770 | 1.770 |
Number of reflections | 47519 | 4671 |
<I/σ(I)> | 12.52 | |
Completeness [%] | 97.4 | |
Redundancy | 6.1 | |
CC(1/2) | 0.996 | 0.765 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 283 | Mg(OAc)2, PEG 3350, bis-tris |