7VMH
Crystal structure of Arabidopsis thaliana HDT4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
Synchrotron site | RRCAT INDUS-2 |
Beamline | PX-BL21 |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2019-07-17 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97947 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 90.482, 73.114, 72.580 |
Unit cell angles | 90.00, 96.86, 90.00 |
Refinement procedure
Resolution | 44.960 - 1.850 |
R-factor | 0.1948 |
Rwork | 0.193 |
R-free | 0.22843 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7vmf |
RMSD bond length | 0.014 |
RMSD bond angle | 1.686 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.290 | 1.890 |
High resolution limit [Å] | 1.850 | 1.850 |
Number of reflections | 38947 | 2203 |
<I/σ(I)> | 10.9 | |
Completeness [%] | 96.9 | 88.6 |
Redundancy | 3.7 | 3.7 |
CC(1/2) | 0.997 | 0.819 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.2 M l-proline, 0.1 m HEPES pH 7.5, 24% W/V PEG 1500 |