7VMF
Crystal structure of Arabidopsis thaliana HDT2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 103 |
| Detector technology | PIXEL |
| Collection date | 2017-07-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.07227 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 53.106, 96.221, 196.299 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.770 - 1.320 |
| R-factor | 0.1616 |
| Rwork | 0.160 |
| R-free | 0.18915 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6j2z |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.710 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.070 | 1.340 |
| High resolution limit [Å] | 1.320 | 1.320 |
| Number of reflections | 111734 | 4443 |
| <I/σ(I)> | 13.9 | 2.1 |
| Completeness [%] | 95.0 | 77.2 |
| Redundancy | 3.6 | 2.4 |
| CC(1/2) | 0.999 | 0.853 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 0.2M ammonium phosphate dibasic pH-8.0, 20%W/V PEG 3350 |
