7VM8
Crystal structure of the MtDMI1 gating ring
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2019-10-10 |
Detector | MAR555 FLAT PANEL |
Wavelength(s) | 0.9793 |
Spacegroup name | I 4 |
Unit cell lengths | 110.982, 110.982, 91.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.800 - 3.034 |
R-factor | 0.2626 |
Rwork | 0.260 |
R-free | 0.31930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6o6j |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 10.000 | 3.110 |
High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
Rmerge | 0.087 | 0.039 | 0.975 |
Rmeas | 0.090 | 0.040 | 0.900 |
Rpim | 0.025 | 0.011 | 0.289 |
Number of reflections | 14703 | 1108 | 1087 |
<I/σ(I)> | 7.1 | ||
Completeness [%] | 100.0 | 99.8 | 100 |
Redundancy | 13.1 | 12.7 | 12.3 |
CC(1/2) | 0.999 | 0.853 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.4 | 293 | 16% (wt/vol) PEG 4000, 0.2M KCl, 0.01M CaCl2, 0.05 M Sodium cacodylate trihydrate pH 5.4 |