7VM0

Crystal structure of YojK from B.subtilis in complex with UDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL18U
Synchrotron site	NFPSS
Beamline	BL18U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	46.602, 81.053, 100.676
Unit cell angles	90.00, 91.87, 90.00

Refinement procedure

Resolution	42.780 - 1.900
R-factor	0.1999
Rwork	0.198
R-free	0.24270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6kqx
RMSD bond length	0.009
RMSD bond angle	1.659
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.930
High resolution limit [Å]	1.900	5.160	1.900
Rmerge	0.085	0.056	0.530
Rmeas	0.092	0.061	0.573
Rpim	0.035	0.024	0.215
Total number of observations	401222
Number of reflections	58223	2955	2911
<I/σ(I)>	6
Completeness [%]	98.9	97.4	99.5
Redundancy	6.9	6.4	7
CC(1/2)		0.996	0.898

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	0.09 M magnesium chloride, 0.09 M Tris-HCl, pH8.0, 25.2% (w/v) PEG3,350 and 0.05M sodium fluoride