7VL7
The complex structure of beta-1,2-glucosyltransferase from Ignavibacterium album with esculin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-11-21 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 163.494, 71.705, 129.817 |
Unit cell angles | 90.00, 105.82, 90.00 |
Refinement procedure
Resolution | 19.876 - 1.890 |
Rwork | 0.174 |
R-free | 0.21460 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.544 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.900 | 1.920 |
High resolution limit [Å] | 1.890 | 1.890 |
Rmerge | 0.117 | 0.551 |
Number of reflections | 115794 | 5660 |
<I/σ(I)> | 6.9 | 1.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M Tris-HCl (pH 7.5), 0.2M calcium acetate, 20%(w/v) PEG 3350 |