7VKL
Crystal structure of MmIMP3-KH12 in complex with zipcode RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-09-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97915 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.982, 62.710, 65.687 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.300 - 1.950 |
R-factor | 0.2146 |
Rwork | 0.212 |
R-free | 0.25680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7EYZ |
RMSD bond length | 0.007 |
RMSD bond angle | 0.920 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.128 | 1.078 |
Rmeas | 0.133 | 1.139 |
Rpim | 0.038 | 0.361 |
Number of reflections | 11985 | 1169 |
<I/σ(I)> | 23.167 | 2.2 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 12.2 | 9.5 |
CC(1/2) | 0.992 | 0.713 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2 M Lithium sulfate monohydrate, 0.1 M HEPES pH 7.5, 25% w/v Polyethylene glycol 3350 |