7VKJ

Structure of ESRP1 qRRM3 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97911
Spacegroup name	P 21 21 21
Unit cell lengths	89.572, 102.813, 107.141
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.347 - 1.450
Rwork	0.171
R-free	0.18990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7vki
RMSD bond length	0.014
RMSD bond angle	1.915
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.500
High resolution limit [Å]	1.450	1.450
Rmerge	0.105	0.966
Rmeas	0.109	1.006
Rpim	0.031	0.278
Number of reflections	2073014	17259
<I/σ(I)>	26.176	3.214
Completeness [%]	93.8	99.6
Redundancy	12.6	12.3
CC(1/2)	0.996	0.876

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Potassium sodium tartrate tetrahydrate, 20% w/v Polyethylene glycol 3350