7VKI
ESRP1 qRRM2 in complex with 12mer-RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.94989 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 86.118, 86.118, 43.280 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.250 - 1.650 |
R-factor | 0.1958 |
Rwork | 0.195 |
R-free | 0.22040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rvj |
RMSD bond length | 0.007 |
RMSD bond angle | 0.864 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.145 | 0.334 |
Rmeas | 0.150 | 0.356 |
Rpim | 0.038 | 0.118 |
Number of reflections | 22615 | 2200 |
<I/σ(I)> | 17.429 | 4.333 |
Completeness [%] | 99.8 | 98.6 |
Redundancy | 11.8 | 7 |
CC(1/2) | 0.981 | 0.933 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.2 M Sodium malonate pH 7.0, 20% w/v Polyethylene glycol 3350 |