7VKC

Crystal structure of a bacterial Ser/Thr kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-12-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	52.948, 64.638, 90.416
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.320 - 1.460
R-factor	0.1678
Rwork	0.167
R-free	0.18480
Structure solution method	SAD
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.320	1.510
High resolution limit [Å]	1.457	1.457
Rmerge		0.100
Number of reflections	50396	1966
<I/σ(I)>	2.2
Completeness [%]	100.0
Redundancy	12.3
CC(1/2)	0.998	0.887

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	8% Tacsimate PH 6.0, 20% PEG 3350