7VKB
Crystal structure of the a bacterial kinase complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-12-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97981 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 45.220, 45.220, 394.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.870 - 1.820 |
| R-factor | 0.2068 |
| Rwork | 0.205 |
| R-free | 0.24600 |
| Structure solution method | SAD |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.871 | 1.870 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.089 | |
| Rpim | 0.091 | |
| Number of reflections | 38842 | 2774 |
| <I/σ(I)> | 2 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 15.1 | |
| CC(1/2) | 1.000 | 0.801 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 289 | 0.1M Sodium acetate PH 7.0 12% PEG 3350 |
