Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-13 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.201, 34.566, 81.299 |
| Unit cell angles | 90.00, 124.58, 90.00 |
Refinement procedure
| Resolution | 39.601 - 1.310 |
| R-factor | 0.1571 |
| Rwork | 0.156 |
| R-free | 0.18480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7CQ9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.954 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2-4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.250 |
| High resolution limit [Å] | 1.230 | 1.230 |
| Number of reflections | 63673 | 3175 |
| <I/σ(I)> | 18.28 | |
| Completeness [%] | 98.9 | |
| Redundancy | 6.3 | |
| CC(1/2) | 0.968 | 0.816 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.2M MgCl2, 0.1M Tris pH 8.5, 20% PEG 8000 |
