7VJN

Crystal structure of anti-CRISPR-associated protein Aca1 in Pseudomonas phage JBD30

Replaces:  7FA2Replaces:  7C0A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-29
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	45.698, 50.435, 64.924
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.829 - 1.799
R-factor	0.1917
Rwork	0.190
R-free	0.21450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7fa3
RMSD bond length	0.013
RMSD bond angle	1.211
Data reduction software	HKL-3000 (v705c)
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.19.2-4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.860
High resolution limit [Å]	1.799	1.800
Rmerge	0.069
Number of reflections	14457	1407
<I/σ(I)>	29.4
Completeness [%]	99.7
Redundancy	10.2
CC(1/2)		0.944

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.2 M di-potassium hydrogen phosphate, 20% (w/v) PEG 3350