7VJ8
SFX structure of archaeal class II CPD photolyase from Methanosarcina mazei in the oxidized state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | SACLA BEAMLINE BL2 |
Synchrotron site | SACLA |
Beamline | BL2 |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2017-11-09 |
Detector | MPCCD |
Wavelength(s) | 1.7712035 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 70.550, 70.550, 245.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.600 - 2.100 |
R-factor | 0.1865 |
Rwork | 0.184 |
R-free | 0.22820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xry |
RMSD bond length | 0.003 |
RMSD bond angle | 0.521 |
Data reduction software | CrystFEL (0.6.3) |
Data scaling software | CrystFEL (0.6.3) |
Phasing software | PHASER (2.8.2) |
Refinement software | PHENIX ((1.19_4092: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.200 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 37362 | 3630 |
<I/σ(I)> | 7.63 | 0.56 |
Completeness [%] | 100.0 | 100 |
Redundancy | 620.8 | 137.9 |
CC(1/2) | 0.990 | 0.570 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 8 | 277 | 0.5-0.65 M Li2SO4, 10-15% (W/V) PEG 8000 |