7VIM

The C-terminal DNA binding domain of EsrB from Edwardsiella piscicida

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979260
Spacegroup name	P 21 21 21
Unit cell lengths	39.111, 58.051, 81.043
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.193 - 2.200
R-factor	0.2316
Rwork	0.229
R-free	0.28070
Structure solution method	AB INITIO PHASING
RMSD bond length	0.008
RMSD bond angle	1.112
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.193	47.193	2.310
High resolution limit [Å]	2.188	6.920	2.190
Rmerge		0.043	0.643
Rmeas	0.084	0.047	0.702
Rpim	0.034	0.020	0.278
Total number of observations	62270	2053	8294
Number of reflections	9987	379	1368
<I/σ(I)>	11.4	28.6	3
Completeness [%]	99.4	99.8	96.1
Redundancy	6.2	5.4	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	12% ethanol, 5% glycerol, 100mM Tris PH 8,5