7VID

The crystal structure of L-leucine dehydrogenase from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9794
Spacegroup name	P 21 21 2
Unit cell lengths	142.620, 90.180, 61.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	76.220 - 2.500
R-factor	0.1983
Rwork	0.196
R-free	0.24860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1leh
RMSD bond length	0.008
RMSD bond angle	0.968
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MrBUMP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.200	2.600
High resolution limit [Å]	2.500	2.500
Rmeas	0.160	1.420
Number of reflections	28028	4015
<I/σ(I)>	13.4	2.5
Completeness [%]	99.8
Redundancy	11.6
CC(1/2)	0.998	0.714

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	12 % (w/v) polyethylene glycol 3,350, 0.1M sodium acetate trihydrate