7VIB
Crystal structure of human ACE2 and GX/P2V RBD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL10U2 |
Synchrotron site | SSRF |
Beamline | BL10U2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-26 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 81.672, 120.150, 107.885 |
Unit cell angles | 90.00, 96.61, 90.00 |
Refinement procedure
Resolution | 47.290 - 3.200 |
R-factor | 0.2072 |
Rwork | 0.204 |
R-free | 0.25790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6m0j |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.260 |
High resolution limit [Å] | 3.200 | 8.670 | 3.200 |
Rmerge | 0.107 | 0.021 | 0.713 |
Rmeas | 0.125 | 0.025 | 0.829 |
Rpim | 0.064 | 0.013 | 0.417 |
Number of reflections | 50664 | 1732 | 1713 |
<I/σ(I)> | 5.2 | ||
Completeness [%] | 98.4 | 97.8 | 99.9 |
Redundancy | 3.7 | 3.5 | 4 |
CC(1/2) | 0.988 | 0.756 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 290 | 20%(w/v) PEG3000 100 mM CAPS pH10.5 200 mM Nacl 10 mM CaCl2 |