7VHR
Apostichopus japonicus ferritin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.55005 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 126.820, 178.780, 126.710 |
| Unit cell angles | 90.00, 90.26, 90.00 |
Refinement procedure
| Resolution | 49.660 - 2.756 |
| R-factor | 0.1932 |
| Rwork | 0.190 |
| R-free | 0.26470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5wpn |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.410 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.800 |
| High resolution limit [Å] | 2.750 | 7.460 | 2.750 |
| Rmerge | 0.123 | 0.043 | 0.557 |
| Rmeas | 0.146 | 0.050 | 0.675 |
| Rpim | 0.077 | 0.027 | 0.374 |
| Total number of observations | 494454 | ||
| Number of reflections | 142777 | 7314 | 6811 |
| <I/σ(I)> | 6.6 | ||
| Completeness [%] | 98.4 | 99.1 | 94.7 |
| Redundancy | 3.5 | 3.5 | 3.1 |
| CC(1/2) | 0.997 | 0.751 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | Magnesium acetate tetrahydrate, Sodium cacodylate trihydrate, 2-Methyl-2,4-pentanediol |
