7VHF
Crystal structure of the STX2a complexed with RRA peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2018-02-04 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.1000 |
Spacegroup name | P 61 |
Unit cell lengths | 146.171, 146.171, 60.514 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.850 - 1.750 |
R-factor | 0.167 |
Rwork | 0.166 |
R-free | 0.18600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7d6r |
RMSD bond length | 0.006 |
RMSD bond angle | 0.848 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.850 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Number of reflections | 74551 | |
<I/σ(I)> | 20.9 | |
Completeness [%] | 100.0 | |
Redundancy | 20.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 4 M Sodium Formate, 100mM MES pH 6.5, 50 mM PPS |