7VH4

Crystal structure of oligoribonuclease of Escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-05-15
Detector	PSI JUNGFRAU 4M
Wavelength(s)	0.96863
Spacegroup name	P 41 21 2
Unit cell lengths	100.574, 100.574, 147.831
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.630 - 2.300
R-factor	0.1963
Rwork	0.194
R-free	0.24490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2igi
RMSD bond length	0.008
RMSD bond angle	1.422
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.630	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.074	0.533
Rmeas	0.086	0.615
Rpim	0.043	0.299
Number of reflections	34389	3274
<I/σ(I)>	11.7	2.9
Completeness [%]	99.8	98.6
Redundancy	7.8	8
CC(1/2)	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	20 mM Sodium HEPES, pH 7.5, 0.25 M Sodium acetate, 25 % v/v PEG-3350