7VGO

Hen egg lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-12
Detector	ADSC QUANTUM 210
Wavelength(s)	1.000
Spacegroup name	P 43 21 2
Unit cell lengths	78.816, 78.816, 36.917
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.490 - 1.200
R-factor	0.1455
Rwork	0.145
R-free	0.16160
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4qeq
RMSD bond length	0.005
RMSD bond angle	0.878
Data reduction software	HKL-2000 (2.3.10)
Data scaling software	HKL-2000 (2.3.10)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.220
High resolution limit [Å]	1.200	1.200
Number of reflections	36189	1522
<I/σ(I)>	66.38	6.8
Completeness [%]	97.9	84.3
Redundancy	12.9	6.28
CC(1/2)		0.941

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.7	293	0.1M sodium acetate, 8%(w/v) sodium chloride, 0.1M cesium chloride