7VGL
Crystal structure of CmnC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-06-12 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 92.946, 127.400, 139.265 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.870 - 1.670 |
R-factor | 0.1804 |
Rwork | 0.179 |
R-free | 0.20990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wbq |
RMSD bond length | 0.006 |
RMSD bond angle | 1.294 |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.730 |
High resolution limit [Å] | 1.670 | 1.670 |
Rmerge | 0.055 | 0.760 |
Number of reflections | 95108 | 9456 |
<I/σ(I)> | 24.04 | 2 |
Completeness [%] | 99.9 | |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M Ammonium acetate, 0.1M Na citrate tribasic dihydrate pH 5.5, 24% v/v Polyethylene glycol 400 |