7VFR
GltA N83K mutant from Bifidobacterium infantis JCM 1222 complexed with lacto-N-tetraose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-25 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 |
Unit cell lengths | 39.995, 42.390, 59.605 |
Unit cell angles | 107.05, 102.06, 104.29 |
Refinement procedure
Resolution | 38.290 - 1.560 |
R-factor | 0.1519 |
Rwork | 0.150 |
R-free | 0.18790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2z8f |
RMSD bond length | 0.010 |
RMSD bond angle | 1.623 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.290 | 1.590 |
High resolution limit [Å] | 1.560 | 1.560 |
Rmerge | 0.076 | 0.169 |
Rmeas | 0.108 | 0.239 |
Rpim | 0.076 | 0.169 |
Number of reflections | 42429 | 1366 |
<I/σ(I)> | 10.1 | 2.8 |
Completeness [%] | 86.5 | |
Redundancy | 1.6 | |
CC(1/2) | 0.940 | 0.920 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293.15 | 30% (w/v) PEG MME 550, 10mM ZnSO4, 0.1M MES-NaOH (pH 5.5) |