7VFF
Cryo-EM structure of Vaccinia virus scaffolding protein D13 with N-terminal 17 residue truncation
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TALOS ARCTICA |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.1 |
| Number of Particle | 743834 |
| Software | PHENIX,RELION,EPU,CTFFIND,UCSF Chimera,Coot |
| Symmetry | C3 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
