7VF4

Crystal structure of Vps75 from Candida albicans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	83.610, 137.170, 226.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.750 - 3.100
R-factor	0.2468
Rwork	0.244
R-free	0.29220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3q66
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MoRDa
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.210
High resolution limit [Å]	3.100	6.670	3.100
Rmerge	0.112	0.056	1.365
Rmeas	0.117	0.059	1.422
Rpim	0.033	0.017	0.394
Total number of observations	613898
Number of reflections	47803	5059	4665
<I/σ(I)>	5.6
Completeness [%]	99.2	98.9	98.8
Redundancy	12.8	11.6	12.7
CC(1/2)		0.999	0.802

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	0.05M cesium chloride, 0.1M MES monohydrate pH 6.5, 30% (v/v) Jeffamine M-600