7VEX

Crystal Structure of GTP-bound Irgb6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-05
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	69.618, 75.743, 80.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.290 - 1.510
R-factor	0.14831
Rwork	0.147
R-free	0.19687
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1tqd 1tpz 1tq2
RMSD bond length	0.013
RMSD bond angle	1.768
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.290	1.600
High resolution limit [Å]	1.510	1.510
Number of reflections	129901	20925
<I/σ(I)>	15.07
Completeness [%]	99.9
Redundancy	6.99
CC(1/2)	0.999	0.524

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Sodium Citrate, Polyethylene Glycol 3350