7VEV
Crystal structure of bacterial chemotaxis-dependent pectin-binding protein SPH1118 in complex with MES
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-01-31 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 49.060, 54.210, 57.012 |
Unit cell angles | 75.86, 66.90, 87.95 |
Refinement procedure
Resolution | 26.133 - 1.498 |
Rwork | 0.167 |
R-free | 0.18790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ipw |
RMSD bond length | 0.014 |
RMSD bond angle | 1.717 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.520 |
High resolution limit [Å] | 1.498 | 1.500 |
Rmerge | 0.055 | 0.139 |
Number of reflections | 79704 | 10578 |
<I/σ(I)> | 27.4 | |
Completeness [%] | 99.9 | |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 20% PEG6000 0.2 M Calcium chloride 0.1 M MES |