7VES

Crystal Structure of nucleotide-free Irgb6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-25
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	41.156, 63.223, 155.821
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.130 - 2.000
R-factor	0.2099
Rwork	0.207
R-free	0.24680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1tqd 1tpz 1tq2
RMSD bond length	0.009
RMSD bond angle	0.816
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.130	2.000
High resolution limit [Å]	1.890	1.890
Number of reflections	61062	8033
<I/σ(I)>	9.63
Completeness [%]	96.5
Redundancy	4.62
CC(1/2)	0.997	0.422

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	MIB buffer, Polyethylene Glycol 1500