7VER
Crystal structure of bacterial chemotaxis-dependent pectin-binding protein SPH1118 in a full open conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-18 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 79.961, 84.727, 90.025 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.848 - 1.699 |
Rwork | 0.180 |
R-free | 0.21190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7vev |
RMSD bond length | 0.014 |
RMSD bond angle | 1.697 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.848 | 1.800 |
High resolution limit [Å] | 1.699 | 1.700 |
Rmerge | 0.045 | 0.253 |
Number of reflections | 66777 | 9726 |
<I/σ(I)> | 33.3 | |
Completeness [%] | 98.3 | |
Redundancy | 10.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 25% PEG550MME 0.1 M MES 0.01 M Zinc sulfate 0.0209 mM Polygalacturonic acid sodium |