7VEP
Crystal structure and biophysical characterization of TPR domain of EccA5 from ESX-5 pathway of Mycobacterium tuberculosis H37RVR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
Synchrotron site | RRCAT INDUS-2 |
Beamline | PX-BL21 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-04-17 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.236, 65.645, 98.204 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.319 - 2.150 |
R-factor | 0.1877 |
Rwork | 0.186 |
R-free | 0.21640 |
Structure solution method | SAD |
Data reduction software | XDS (0.70) |
Data scaling software | Aimless (7.0.072) |
Phasing software | PHENIX (2.8.2) |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.140 | 2.200 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.093 | 0.631 |
Rmeas | 0.116 | 0.793 |
Rpim | 0.067 | 0.474 |
Number of reflections | 20585 | 1717 |
<I/σ(I)> | 2.3 | 2.3 |
Completeness [%] | 99.2 | 98.3 |
Redundancy | 5.3 | 4.7 |
CC(1/2) | 0.997 | 0.715 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 300 | Lithium sulfate, sodium citrate 5.6, Ammonium sulfate, glycerol as additive |