7VEM

the NADPH-assisted quinone oxidoreductase from Phytophthora capsici

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 32 2 1
Unit cell lengths	84.281, 84.281, 185.605
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	72.990 - 2.390
R-factor	0.1703
Rwork	0.167
R-free	0.22420
Structure solution method	SAD
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	SOLVE
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.990	2.440
High resolution limit [Å]	2.390	2.400
Number of reflections	30531	30531
<I/σ(I)>	11.19
Completeness [%]	99.9
Redundancy	9
CC(1/2)	0.966	0.936

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		283	28% v/v 2-Propanol, 0.1M BIS-TRIS pH 6.5, 3% v/v Polyethylene glycol 200