7VEE
The ligand-free structure of GfsA KSQ-AT didomain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-29 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 230.763, 230.763, 117.950 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.530 - 2.550 |
| R-factor | 0.1976 |
| Rwork | 0.196 |
| R-free | 0.23680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mz0 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.427 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.650 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.033 | 0.810 |
| Number of reflections | 39110 | 4388 |
| <I/σ(I)> | 29.2 | 2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.8 | 7 |
| CC(1/2) | 1.000 | 0.859 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.1 | 298 | sodium malonate, glycerol, sodium chloride, HEPES |
