7VEE
The ligand-free structure of GfsA KSQ-AT didomain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-29 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0 |
Spacegroup name | H 3 2 |
Unit cell lengths | 230.763, 230.763, 117.950 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.530 - 2.550 |
R-factor | 0.1976 |
Rwork | 0.196 |
R-free | 0.23680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mz0 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.427 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.650 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.033 | 0.810 |
Number of reflections | 39110 | 4388 |
<I/σ(I)> | 29.2 | 2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.8 | 7 |
CC(1/2) | 1.000 | 0.859 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.1 | 298 | sodium malonate, glycerol, sodium chloride, HEPES |