7VE6

N-terminal domain of VraR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2019-05-17
Detector	RAYONIX MX-300
Wavelength(s)	0.9984
Spacegroup name	P 32 2 1
Unit cell lengths	122.713, 122.713, 67.522
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.930 - 2.770
Rwork	0.178
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4if4
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.930	2.870
High resolution limit [Å]	2.770	2.770
Rmerge	0.055	1.142
Number of reflections	14756	1152
<I/σ(I)>	37.16
Completeness [%]	97.2
Redundancy	9.2	9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	294	100 mM Tris-Hcl pH 8.0, 18% PEG 550MME (w/v), 5% Glycerol, 200 mM MgCl2