7VDN
High resolution crystal structure of Sperm Whale Myoglobin in the carbonmonoxy form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-20 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54056 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.403, 30.702, 63.804 |
| Unit cell angles | 90.00, 105.56, 90.00 |
Refinement procedure
| Resolution | 20.020 - 0.930 |
| R-factor | 0.1882 |
| Rwork | 0.187 |
| R-free | 0.21050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vxc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.084 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.020 | 0.950 |
| High resolution limit [Å] | 0.930 | 0.930 |
| Rmerge | 0.114 | 0.914 |
| Rmeas | 0.124 | |
| Rpim | 0.047 | |
| Number of reflections | 86393 | 4341 |
| <I/σ(I)> | 9.7 | 0.5 |
| Completeness [%] | 100.0 | 99.7 |
| Redundancy | 11.9 | 5 |
| CC(1/2) | 0.998 | 0.069 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 293 | 2.8 M ammonium sulfate, 50 mM K2HPO4, 50 mM NaH2PO4, 10% glycerol, 2 mg/ml sodium dithionite |
