7VD7
Toxin - Antitoxin complex from Salmonella enterica serovar Typhimurium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-09 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.97959 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.366, 31.605, 47.985 |
Unit cell angles | 90.00, 90.63, 90.00 |
Refinement procedure
Resolution | 24.000 - 1.430 |
R-factor | 0.19664 |
Rwork | 0.196 |
R-free | 0.21615 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | no PDB entry |
RMSD bond length | 0.013 |
RMSD bond angle | 1.730 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.19.2-4158) |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.460 |
High resolution limit [Å] | 1.420 | 1.420 |
Number of reflections | 20736 | 1569 |
<I/σ(I)> | 49.02 | |
Completeness [%] | 93.1 | |
Redundancy | 6.4 | 5.7 |
CC(1/2) | 0.999 | 0.975 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% (w/v) PEG 6000 100 mM HEPES/ Sodium hydroxide pH 7.0 200 mM Sodium chloride |