7VCR
Crystal Structure of PitA fragment from pilus islet-2 of Streptococcus oralis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97951 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.480, 70.875, 82.466 |
| Unit cell angles | 80.09, 87.02, 87.70 |
Refinement procedure
| Resolution | 69.879 - 2.000 |
| Rwork | 0.201 |
| R-free | 0.23390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7vcn |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.925 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.150 | 2.040 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.052 | 0.818 |
| Rpim | 0.047 | 0.647 |
| Number of reflections | 83573 | 4541 |
| <I/σ(I)> | 11.3 | 1.3 |
| Completeness [%] | 90.3 | 92.1 |
| Redundancy | 3.2 | |
| CC(1/2) | 0.999 | 0.727 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 295 | 0.2 M ammonium acetate 0.1 M HEPES pH 7.2, 20% PEG 3350 |
